Puzzle master Professor Layton and his apprentice, Luke, have stumbled upon another mystery. It's up to them to navigate their way through numerous puzzles to determine who - or what - caused the death of Professor Layton's mentor, Dr. Schrader. Was it the mysterious Pandora's Box, rumoured to kill all who open it? Professor Layton and Luke find a clue that begins their adventure: a train ticket for the Molentary Express.